Service for mAb Epitopes Mapping Using Cryo-EM

Service for mAb Epitopes Mapping Using Cryo-EM

Therapeutic monoclonal antibodies (mAbs) play a substantial role in the biopharmaceutical market, accounting for more than 50% of therapeutic protein sales, and experiencing continuous growth. In the development of new vaccines, therapeutic antibodies, and diagnostic reagents, it is critical to select mAbs with the appropriate affinity, specificity, and biophysical properties.

 

Applications of Cryo-EM-Based mAb Epitopes Mapping

1.Reveal the true structure of the target protein.

 

2.Explain the differences between various antibodies based on the structure of the antigen-antibody complexes.

 

3.Identify the spatial epitope information of antibody recognition, which can help antibody drugs apply for patents and expand the scope of market protection.

 

4.Reveal the dynamic and complex structural and functional mechanisms of the antigen-antibody during the entire immune response, facilitating the optimized design of next-generation immunotherapies targeting the protein.

 

Why Choose CryoEM-Solutions

1.No need for crystallization of antigen-antibody complexes

This service is broadly applicable to large molecular weight antigen proteins, multi-pass transmembrane proteins, and proteins rich in glycosylation modifications.

 

2.No need for sequence truncation or modification of proteins

Epitope mapping can be based on full-length antigen proteins that are stable in detergents or assembled in lipid nanodiscs.

 

3.Closer to the spatial structure of the real internal environment

Cryo-EM allows for the resolution of complex dynamic structures or oligomeric structures of antigen-antibody complexes in a near-natural, fully hydrated state. This offers new avenues for revealing the mechanisms of action of some successful therapeutic monoclonal antibodies, aiding in the optimized design of next-generation immunotherapies targeting various disease antigens.

 

Services at CryoEM-Solutions