Protein MicroED Three-Dimensional Structure Analysis Service
Protein MicroED Three-Dimensional Structure Analysis Service
Microelectron Diffraction (MicroED) represents the advanced technology for the 3D reconstruction of organic small molecules and biomacromolecules, such as proteins, utilizing electron diffraction data from Cryo-Transmission Electron Microscopy (Cryo-TEM). As a cutting-edge methodology, MicroED uses electrons as the incident beam to acquire microcrystal electron diffraction data from Cryo-TEM. Similar to diffraction methods like X-ray crystallography, MicroED requires the crystallized sample. Although the crystallization process is largely analogous to X-ray crystallography, the requisite crystal size for MicroED is much smaller, in the order of hundreds of nanometers. MicroED technology enables rapid and high-resolution determination of the three-dimensional structure of small molecule compounds and biological macromolecules, minimizing scientific research time and costs.
Workflow
1.Sample preparation and delivery
1) Protein crystal sample preparation and delivery
A. micro-crystalline glass samples preparation
B. focused ion beam(FIB) cutting
2) Small molecule powder sample preparation and delivery
2.Diffraction data collection
3.Structure elucidation
The Applications of Protein MicroED Three-Dimension Structure Analysis
1.Identification of the structure and crystal form of small molecule drugs.
2.Analyzing crystals samples that are not large enough to be analyzed by X-ray crystallography.
Why Choose CryoEM-Solutions
At Cryo-EM Solutions,We leverage our core technologies to propel digital innovation and facilitate global pharmaceutical enterprises significantly improve the efficiency and success rate of drug discovery and development. Besides, we can also provide you with high-quality MicroED structural analysis services:
1.Easy sample preparation
Only a small amount of simple powder samples and needle-pierced crystallites are required, which greatly expands the application range of electron microscopy technology.
2.High-Throughput process
The sample preparation process is simple, fast and high-throughput. The electron microscope operation time is short, with data collection for a single sample taking less than 30 minutes.
3.Extremely High-Resolution data
Crystals below 200 nm can be easily analyzed. With a limiting resolution of less than 1 Å, molecular structures can be obtained at atomic resolution, and even conformational analysis can be performed for partial groups.
Services at CryoEM-Solutions
