High-Throughput X-ray Protein Crystallography Screen Service
High-Throughput X-ray Protein Crystallography Screen Service
X-ray crystallography is one of the most widely used techniques by structural biologists. As an important tool for revealing the chemical properties of crystals and the 3D structure of molecules, X-ray protein crystallography contributes a lot to the development of Protein Data Bank (PDB). PDB has collected more than 170,000 biological structural information of macromolecules. About 90% of the biological macromolecule atomic coordinate data is derived from X-ray crystallography studies. This enables scientists to have a profound insight into the atomic or near-atomic resolution structures of macromolecules. This, in turn, deepens our understanding of biochemical and molecular processes, and elucidates the spatial distribution of atoms in biological phenomena.
High-throughput crystal screening and detection services rely on an automated crystal platform comprising the Mosquito Crystal and UV fluorescence imaging system. Mosquito crystal is an automatic device for protein crystal screening, which can greatly improve the efficiency of the experiment with a comprehensive crystal screening kit. The UV fluorescence imaging system serves as specialized equipment for observing protein crystal growth through UV fluorescence, helping scientists to efficiently discover and acquire protein crystals.
Workflow
1.Sample preparation
2.Crystal screening
3.Protein crystal identification
4.Data collection
5.Structure analysis
Applications of X-ray Protein Crystallography
1.Resolve the 3D structure of crystallizable proteins and their complexes.
2.Characterize the spatial epitopes of antigen-antibody interactions.
3.Verify the structure-activity relationship of small molecule-protein interactions.
Why Choose CryoEM-Solutions
1.High-Throughput and automation
Cryo-EM Solutions use automated equipment combined with high-performance detection systems to greatly improve experimental efficiency, making crystal screening experiments faster, cheaper, and simpler than ever before.
2.Full coverage of protein crystallization experiments
Cryo-EM Solutions conducted all kinds of protein crystallization experiments, including suspension, sitting, and oil droplets.
3.Minimal sample usage
Using mosquito crystal for miniaturized protein crystallization screening not only needs just trace amounts of protein samples, but also employs disposable needles to prevent cross-contamination.
Services at CryoEM-Solutions