Cryo-EM Protein-Small Molecule Complex Structure Analysis Service

Cryo-EM Protein-Small Molecule Complex Structure Analysis Service

Structure analysis of protein-small molecule complex means that the target protein or its homologous protein has a high-resolution structure in the PDB database. It is more straightforward to analyze the structure of such proteins (including protein mutants, drug target proteins bound to small molecule compounds, protein-protein complexes, and protein-nucleic acid complexes, etc.).

 

Workflow

1.Sample preparation and delivery

2.Negative staining and preliminary screening

3.Cryo-sample preparation

4.Sample screening and data collection

5.2D classification and initial data processing

6.Structural analysis

7.Model building

 

The Application of Protein Small Molecule Complexes Structure Analysis

1.Explain the differences between different small molecules in terms of the structure of protein-small molecule complexes.

 

2.Identify the spatial epitope information of protein-small molecule complexes, apply for patent protection for small molecule drugs, and extend the market protection period.

 

3.Uncover the complex structure and functional mechanisms involved in the ligand binding process of target protein small molecule complexes, and promote the development of next-generation small molecule drugs.

 

Why Choose CryoEM-Solutions

1.Small molecule computational platform

Cryo-EM Solutions utilizes computational chemistry screening libraries of druggable molecules to provide screening, design, synthesis and activity testing of small molecule compounds and other related services.

 

2.Reduced graphene oxide support film

2.The reduced graphene oxide grid is suitable for biomacromolecules with low concentration (around 100 nanomolar), sensitive properties, small molecular weight (as small as 100kDa) with serious orientation problems, which can utilize the advantages of Cryo-EM Grids.

 

3.Easy sample preparation

There is no need to crystallize the sample, and the frozen vitreous sample can be prepared directly on the machine.

 

4.Minimal sample requirements

Typically, 100 µL of protein sample with a concentration of 2 mg/mL and a purity greater than 95% is suffient.

 

Services at CryoEM-Solutions